Tuesday, April 24, 2012

transport system

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A.


Materials are transported to and from individual cells in organisms. There are various mechanisms through which this transportation occurs, some of which include osmosis, active transport, mass flow, cytoplasmic streaming, and endocytosis. However transport within flowering plants is of nutrients (carbon dioxide, water and essential ions) oxygen, and other elaborated foods. Water and Ions are transported in the xylem vessels, carbon dioxide and oxygen diffuse in to the system and elaborated foods such as sugar and amino acids are transported by the phloem tissues.


In flowering plants water travels through distinct routes. These routes are discussed below


Apoplast


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This pathway consists of spaces within the cellulose cell walls. The volume of these free spaces is known as free space. It includes the water filled spaces of dead cells, hollow tubes of the xylem vessels and intermolecular spaces in the cell walls. This pathway avoids contents of the living cell and as such water traveling through this system meets little resistance. 0% of water in the plant travels through this pathway.


Symplast


The symplast is a pathway through the living contents of the cells, which is the cytoplasm. Water travels via the cytoplasmic connections called plasmodesmata. This route presents considerable resistance as water comes into contact with organelles and membranes.


The vascular route


Here water moves in and out of vacuoles passively by the help of the osmosis through a semi permeable membrane. It is the route by which individual cells absorb water from the apoplast.


Transpiration


Only a minute fraction of water is taken up to the aerial system is retained there or used for photosynthesis and growth. The bulk of the water moving through the plant evaporates from the surface of the cell inside the leaf and escapes the leaves as water vapor. Thus the evaporation of water from the aerial parts of the plant (leaves) is known as transpiration.


A continuous column of water extends from the external surface of the mesophyll cells throughout the free spaces of the plant cell walls, to the water inside the xylem vessels. Due to its cohesive properties, water is drawn up the xylem to replace the water that has evaporated from the walls of the mesophyll cells in the leaves. Thus transpiration maintains a water potential gradient by which water moves from the soil into the root hairs, and across the cortex of the root to the central vascular tissue.


Water can also travel via the root. The roots have hairs, which help increase the surface area. The root hairs grow out to make intimate contact with the film of soil water. In the root water converges onto the central vascular tissue enclosed by the endodermis. Entry into this central region is through the cells of the endodermis. Endodermial cells have a strip of waxy substance consisting of suberin, forming the casparian strip, in their radial walls. This strip prevents water movement via the cell walls, and so all water and solutes moving across the root to the central vascular tissues must pass through the cytoplasm of the endodermal cells.


Tension generated in the leaves causes water to be pushed up by a force generated in the root called root pressure. This is as a result of the vascuolar pathway of water through the epidermis, which causes the center of the root to function as a simple osmomemter. The soil solution is normally very dilute. It has higher water potential than the water inside the root stele. As a result water crosses into the stele by osmosis, and creates the root pressure.


The xylem vessel is very important in the transportation of water and mineral salts. It has a lignified cell wall, which enables it to resist compression and tension. The vessel is under tension because water is drawn up the stem as a result of the reduction in pressure in the leaves when evaporation occurs from the mesophyll cells. The thickening in xylem vessels helps to resist the tendency for the vessels to collapse under tension.


B.


Sugar is manufactured in the leaves by photosynthesis and transported to other parts of the plant where it is used in growth, development and repair or starch storage. Amino acids are moved from cells where they are synthesized to places in the plant where proteins are being synthesized. Sugars and amino acids are transported in the phloem tissue and movement via the phloem tissue is known as translocation.


The phloem consists of sieve tubes and companion cells. Theses usually occur with parenchyma and fibers. These cells known as sieve tube elements are joined end to end to form a system of tubes that intertwine round the whole plant. The end walls and sieve plates are perforated by pores. The companion cells service and maintain the sieve tube elements in various ways.


Evidence that phloem is the site of sugar transport came from bark-ringing experiments and from laborious analyses of the changes in sugar content of xylem, phloem and leaf tissue over 4 hour periods. Plant physiologists have exploited the techniques of radioactive tracing with labeled metabolites, and direct sampling of the contents of individual sieve tubes using the mouthparts of aphids as micropipettes.


Sugar is loaded into the phloem sieve tube via transfer cells. A secondary pump mechanism is involved. Specialized parenchyma cells with an expanded internal surface area facilitate movement across cell membranes. Solutes in the phloem sieve tubes at 5-100cm per hour, faster than diffusion. This is not passive as energy from respiration is required.


Several factors can help the explanation of translocation in the phloem such as the pressure flow hypothesis, cytoplasmic streaming and electro-osmosis. The principle of the pressure flow hypothesis is that solute move down a gradient of hydrostatic pressure. The sieve tubes in a leaf are a source region for pressure flow, while the root cells are a sink region. Hydrostatic pressure between source and sink drives a mass flow of water and dissolved solutes. Sugar formed in the source tissue gives rise to this gradient.


The energy from metabolism required for phloem transport may be invested by companion cells in withdrawing ions such as potassium, from the sieve tube on the side of a sieve plate and secreting them on the other side, creating a potential difference across the plate. Polar water molecule would then be swept along with the stream of ions drawn through the pores by the potential difference. This stream would carry solutes present in thee sieve tube.





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